The presence of sulphur compounds in natural gas can interfere to the quality of natural gas. The decline of combustion gas capacity, metal instrument corrosion in gas piping, and the environmental pollution from gas emission can affect by their presence. Bio-filter is one of the methods that selected to reduce sulphur content in natural gas. A lab scale study of hydrogen sulphide reduction in natural gas had conducted in oil and gas field using bio-filter method. The bio-filter system (±1 L volume) contains an active carbon and thiosulphide medium as a substrate, Thiobacillus thioparus as a single culture of sulphur bacteria, and Thiobacillus thioparus with sludge as a mixed culture of sulphur bacteria. The study of hydrogen sulphide reduction was conducted with continuous flow line process. The gas flow rate approximately 1.5 L/min with a fluctuate presence of Hydrogen sulphide (approximately 40 – 70 mg/L). The bio-filter system contains active carbon, thiosulphide medium, and single culture of T. thioparus appear as a good filter for hydrogen sulphide reduction. During 24 hours, the hydrogen sulphide reduction obtains 93% to 16%. When culture media added, the hydrogen sulphide reduction will increase almost 60% and then the reduction decrease to 4% after 20 hours. It is concluded that the bio-filter have potential to develop for sulphur reduction in natural gas.


Cho, K.S., Zang, L., Hirai, M., Shoda, M., 1991. Removal characteristics of hydrogen sulphide and methanethiol by Thiobacillus sp. Isolated from peat in biological deodourisation. Journal of Fermentation and Bioengineering 71, 44-49.

Ryu, H.W., 1996. Microbial desulphurization of a bituminous coal by iron-oxidazing bacteria Thiobacillus ferrooxidans. Korean Journal of Biotehnology and Bioengineering 11, 238-245.

Chung, Y.C., Huang, C., Tseng, C.P., 1999a. Biodegradation of hydrogen sulphide by a laboratory-scale immobilized Pseudomonas putida CH11 Biofilter. Biotechnology Progress 12, 773- 778.

Chung, Y.C., Huang, C., Tseng, C.P., 1999b.Kinetics of hydrogen sulphide oxidation by immobilized autotrophic bacteria in bioreactors. Biotechnology Techniques 10, 743-748.

Hallberg, K.B., and Borje, E., 1994. Characterization of Thiobacillus caldus sp. Nov., a moderately Thermophilic acidophile, Mycrobiology, vol. 140 part 12.

Oh, K.J., Kim, D., Lee, I.-H., 1998. Development of effective hydrogen sulphide removing equipment using Thiobacillus sp. IW. Environmental Pollution 99, 87-92.

Udiharto, M., 1997a. Desulphurization of petroleum using Thiobacillus neapolitanus, LEMIGAS Scientific Contributions, 2 / 1997.

Suzuki, I., et al, 1992. Oxidation of elemental sulphur and sulphide by Thiobacillus thiooxidans. Cell Applied and Environmental Microbiology, Nov., p 3767-3769.

Udiharto, M., 1997b. Desulfurisasi dalam minyak bumi dan medium oleh Thiobacillus thioparus. Proceeding Temu Karya Pengolahan, Jakarta.

Brock, T.D., Madigan, M.T., 1991. Biology of Microorganisms, 6th Edition. Prentice-Hall, Englewood Cliff, NJ.

Huang, C., Chung, Y.C., Hsu, B.M., 1996. Hydrogen sulphide removal by immobilized autotrophic ang heterotrophic bacteria in the bioreactors. Biotechnology Techniques 10, 595-600.

Cha, J.M., Park, Y., Lee, I.W., 1994. Effect of cultivation condition on growth of hydrogen sulphide-degrading Thiobacillus sp. IW isolated Biotechnology and Bioengineering 9, 287-293.

Kim, S.M., Oh, K.H., Kim, D., 1996. The immobilized characteristics of Thiobacillus sp. IW. Korean Journal of Biotechnology and Bioengineering 11, 78-85.



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.